How human insulin is produced from biotechnology? (3 marks/5 marks)

Answers 1

Answer:

Human insulin is extracted from pancreas cells and an insulin-producing gene is located.

Explanation:

1. A plasmid DNA is extracted from a bacterium and cut with restriction enzyme, forming plasmid vector.

2. Insert human insulin-producing gene into the bacterial plasmid vector to form the recombinant DNA of human insulin-producing gene.

3. Introduce this recombinant DNA into a bacterial cell to form the recombinant bacterium.

4. The recombinant bacteria multiply in a fermentation tank and produce human insulin.

5.Insulin is extracted, purified and bottled. It is then ready to be injected into diabetic patients.

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