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In Gram positive bacteria the reason of this negative charge is the presence of teichoic acids linked to either the peptidoglycan or to the underlying plasma membrane. The charge in the bacterial cell wall is due to the electron release due to the catalytic activity during cell respiration. The Gram negative bacteria have an outer covering of phospholipids and Lipopolysaccharides. The lipopolysaccharides impart a strongly negative charge to surface of Gram negative bacterial cells.
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