Why we use Dyer's method to perform cell wall staining?
Answers 1
In this method, the bacterial cells are treated with Cetylpyridinium chloride solution. In Cetylpyridinium chloride solution Cetylpyridinium chloride is dissolved in water, where it get ionized and formed positively charged and negatively charged ions.
These positively charged ions of Cetylpyridinium get absorbed by the negatively charged cell wall, as result, the cell wall becomes positively charged. When the cell wall is treated with a acidic dye, the congo red, it will stain the cell by reacting with the positively charged cell wall.
As a result of this reaction, a visible red boundary is developed around the bacterial cell due to the selective deposition of staining reagents on the cell wall. Later the cell is treated with a basic dye like methylene blue, as result, the cytoplasm is appeared as blue.
Explanation:
THANKS...
-
Author:
janeviyi
-
Rate an answer:
9