Assertion: Nucleolus is not a membrane bound structure. Reason: The content of nucleolus is continuous with the rest of the nucleoplasm.

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Nucleolus is a part of nuclear matrix. It is not a membrane bounded structure and remains surrounded by nucleoplasm. This means that the nucleolus and the nucleoplasm are in direct contact with each other and they are not separated by any membrane. Hence the content of nucleolus is continuous with the nucleoplasm.

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